4MAH
Structure of Aspergillus oryzae AA11 Lytic Polysaccharide Monooxygenase with Zn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.282 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.207, 61.498, 65.136 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.070 - 1.550 |
R-factor | 0.1337 |
Rwork | 0.132 |
R-free | 0.16560 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.420 |
Data reduction software | XDS |
Data scaling software | SCALA (0.1.29) |
Phasing software | SHELXD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.070 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.052 | 0.370 |
Number of reflections | 32367 | |
<I/σ(I)> | 17.7 | 3.6 |
Completeness [%] | 98.7 | 94.2 |
Redundancy | 5.4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 292 | 10 mM zinc chloride, 100 mM MES, pH 6.0, 20% w/v PEG6000, VAPOR DIFFUSION, temperature 292K |