4MAF
Soybean ATP Sulfurylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 204.268, 230.751, 159.197 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.210 - 2.480 |
R-factor | 0.1751 |
Rwork | 0.173 |
R-free | 0.22150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xnj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.149 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.211 |
High resolution limit [Å] | 2.480 |
Number of reflections | 123420 |
<I/σ(I)> | 18 |
Completeness [%] | 93.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 277 | 0.1 M HEPES, pH 7.3, 18% PEG-8000, 0.2 M NaCl, 5 mM APS, VAPOR DIFFUSION, HANGING DROP, temperature 277K |