4MAA
The Crystal Structure of Amino Acid ABC Transporter Substrate-binding Protein from Pseudomonas fluorescens Pf-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.236, 65.031, 59.962 |
| Unit cell angles | 90.00, 109.89, 90.00 |
Refinement procedure
| Resolution | 29.436 - 2.000 |
| R-factor | 0.1644 |
| Rwork | 0.163 |
| R-free | 0.19510 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.048 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 26573 | |
| <I/σ(I)> | 14.6 | 6.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.9 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2 M Magnesium Chloride, 0.1 M Tris:HCl pH 8.5 20% (w/v) PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
