4M9V

Zfp57 mutant (E182Q) in complex with 5-carboxylcytosine DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-31
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.8
Spacegroup name	P 1 21 1
Unit cell lengths	36.388, 96.093, 36.408
Unit cell angles	90.00, 113.14, 90.00

Refinement procedure

Resolution	33.459 - 0.969
R-factor	0.1307
Rwork	0.130
R-free	0.14260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gzn
RMSD bond length	0.009
RMSD bond angle	1.815
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.500	1.000
High resolution limit [Å]	0.969	0.969
Rmerge	0.070	0.648
Number of reflections	129957
<I/σ(I)>	11.7	1.4
Completeness [%]	95.7	92.7
Redundancy	2.6	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	289	35% 2-methyl-2,4-pentanediol, 15% polyethylene glycol 8000, 100 mM CaCl2, and 100 mM acetate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K