4M9P
Crystal structure of the human filamin A Ig-like domains 3-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976250 |
| Spacegroup name | P 65 |
| Unit cell lengths | 63.041, 63.041, 136.604 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.650 - 1.720 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2NQC AND 3V8O CHAIN A |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.967 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC (refmac_5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.650 | 1.760 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.093 | 0.635 |
| Number of reflections | 32462 | |
| <I/σ(I)> | 18.24 | 3.3 |
| Completeness [%] | 99.7 | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.1 M sodium malonate, 12% w/v PEG3350, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
