4M98
Acetyltransferase domain of PglB from Neisseria gonorrhoeae FA1090
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2012-04-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 86.219, 86.219, 86.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.558 - 1.670 |
| R-factor | 0.1681 |
| Rwork | 0.167 |
| R-free | 0.18820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bsw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.133 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.560 |
| High resolution limit [Å] | 1.670 |
| Number of reflections | 25105 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | sodium acetate pH 4.6, 20mM calcium chloride, 30% 2-methyl-2,4-pentanediol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
