4M94

d(ATCCGTTATAACGGAT) complexed with Moloney Murine Leukemia virus reverse transcriptase catalytic fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OTHER
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-27
Detector	APEX II CCD
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	53.957, 145.722, 46.876
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.730 - 2.140
R-factor	0.202
Rwork	0.201
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.070
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	33.730
High resolution limit [Å]	2.140
Rmerge	0.047
Number of reflections	19858
<I/σ(I)>	21.7
Completeness [%]	93.7
Redundancy	3.75

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	7% PEG 4000, 5mM Magnesium acetate, 50mM N-(2-acetamido)iminodiacetic acid, pH 6.5, vapor diffusion, hanging drop, temperature 293.15K