4M8Z

Crystal Structure of SFH3, a phosphatidylinositol transfer protein that integrates phosphoinositide signaling with lipid droplet metabolism

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97820
Spacegroup name	P 21 21 21
Unit cell lengths	52.521, 114.716, 144.694
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.427 - 1.928
R-factor	0.2187
Rwork	0.218
R-free	0.25070
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.134
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SGXPRO
Refinement software	PHENIX (dev_1423)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	89.890	30.000	2.000
High resolution limit [Å]	1.930	4.150	1.930
Rmerge	0.073	0.048	0.283
Number of reflections	66699
<I/σ(I)>	10.8
Completeness [%]	99.9	99.6	99.9
Redundancy	5.1	4.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	The reservoir solution consisted of 15 - 30% PEG 4000, 5% glycerol, 100 mM ammonium sulfate, 100 mM ammonium acetate pH 5.6. Thin plate-like crystals were harvested 3 - 4 days after mixing 2 ul protein solution (5 mg/ml) with 2 ul reservoir solution., VAPOR DIFFUSION, SITTING DROP, temperature 293K