4M8S
Crystal structure of 3-ketoacyl -(acyl carrier protein) reductase (FabG) from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.890, 111.270, 117.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.540 - 2.000 |
| R-factor | 0.17453 |
| Rwork | 0.173 |
| R-free | 0.19881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.189 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.360 | 35.360 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Number of reflections | 59818 | ||
| Completeness [%] | 100.0 | 98.5 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296.15 | 250mM Ammonium phosphate dibasic, 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K |
