4M8I
1.43 Angstrom resolution crystal structure of cell division protein FtsZ (ftsZ) from Staphylococcus epidermidis RP62A in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97929 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.335, 52.063, 87.005 |
Unit cell angles | 90.00, 109.64, 90.00 |
Refinement procedure
Resolution | 27.330 - 1.430 |
R-factor | 0.17061 |
Rwork | 0.169 |
R-free | 0.20616 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vo8 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.833 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.450 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.055 | 0.548 |
Number of reflections | 53059 | |
<I/σ(I)> | 23.42 | 2.45 |
Completeness [%] | 95.8 | 91.4 |
Redundancy | 5.1 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 6.7 mg/mL protein in 10 mM Tris-HCl, pH 8.3, 0.25 M sodium chloride, 5 mM BME, crystallization conditions: Classics II G5 (#77): 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, 25% w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |