4M8B
Fungal Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-01-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115869 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.293, 103.401, 73.178 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.250 - 2.610 |
| Rwork | 0.198 |
| R-free | 0.23540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | The starting model was a poor initial model built from MAD phases of a SeMet derivative. |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.012 | 2.750 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.512 | |
| Number of reflections | 19969 | |
| <I/σ(I)> | 9.7 | 1.5 |
| Completeness [%] | 93.6 | 87.9 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2 uL drops were a 1 to 1 mixture of protein with a well solution of 9 mM Calcium chloride, 10 mM Spermine, 50 mM Sodium cacodylate pH 7, 4.5% Isopropanol, 3.5% Ethylene glycol. Cryoprotection involved a 4 to 6 mixture of 25% Glucose/Ethylene Glycol and well solution., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
