4M7R

Crystal structure of the N-terminal methyltransferase-like domain of anamorsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 61
Unit cell lengths	79.508, 79.508, 101.750
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.426 - 1.801
Rwork	0.187
R-free	0.22570
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.031
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: dev_1372))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.430	2.030	1.830
High resolution limit [Å]	1.800	1.980	1.800
Rmerge	0.067	0.210	0.510
Number of reflections	33762
<I/σ(I)>	42.9	13.3	4.09
Completeness [%]	100.0	100	100
Redundancy	10.7	11.2	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	289	0.17M sodium acetate trihydrate, 0.085M sodium cacodylate pH6.5, 25% PEG 8000, 15% Glycerol, EVAPORATION, temperature 289K