4M7O
The crystal structure of a possible an iron-binding (periplasmic solute-binding) protein from Staphylococcus epidermidis ATCC 12228.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915, 0.97938 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.052, 48.173, 44.916 |
| Unit cell angles | 90.00, 102.74, 90.00 |
Refinement procedure
| Resolution | 34.720 - 2.020 |
| R-factor | 0.1827 |
| Rwork | 0.180 |
| R-free | 0.22680 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.991 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.050 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.087 | 0.603 |
| Number of reflections | 20707 | |
| <I/σ(I)> | 20.5 | 1.9 |
| Completeness [%] | 97.3 | 81.8 |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.5 | 289 | 0.1M MgCl2, 0.1M MES:NaOH, 30% (v/v) PEG400, pH 6.5, MICRODIALYSIS, temperature 289K |
