4M7L
Crystal structure of the complex between human tissue factor extracellular domain and antibody 10H10 FAB fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-05-12 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 |
| Unit cell lengths | 116.860, 116.860, 106.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.400 |
| R-factor | 0.24466 |
| Rwork | 0.242 |
| R-free | 0.28906 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uj3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.535 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.105 | 0.583 |
| Number of reflections | 9573 | |
| <I/σ(I)> | 19.7 | 3.4 |
| Completeness [%] | 96.7 | 97.4 |
| Redundancy | 7.4 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293 | 0.1 M CAPS, pH 10.5, 24% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
