4M7L
Crystal structure of the complex between human tissue factor extracellular domain and antibody 10H10 FAB fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2010-05-12 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | I 41 |
Unit cell lengths | 116.860, 116.860, 106.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.400 |
R-factor | 0.24466 |
Rwork | 0.242 |
R-free | 0.28906 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uj3 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.535 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 3.500 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.105 | 0.583 |
Number of reflections | 9573 | |
<I/σ(I)> | 19.7 | 3.4 |
Completeness [%] | 96.7 | 97.4 |
Redundancy | 7.4 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293 | 0.1 M CAPS, pH 10.5, 24% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |