4M7H
Crystal structure of tetrameric fibrinogen-like recognition domain of FIBCD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-30 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 4 |
| Unit cell lengths | 118.560, 118.560, 44.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.917 - 2.000 |
| R-factor | 0.1915 |
| Rwork | 0.183 |
| R-free | 0.20940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jc9 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.1.4) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.917 | 41.917 | 2.110 |
| High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
| Rmerge | 0.066 | 0.044 | 0.214 |
| Total number of observations | 4471 | 16153 | |
| Number of reflections | 41125 | ||
| <I/σ(I)> | 12.6 | 12.9 | 2.9 |
| Completeness [%] | 97.8 | 99.1 | 93.3 |
| Redundancy | 3.2 | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6M Ammonium sulphate, 10% Dioxane, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
