4M7G
Streptomyces Erythraeus Trypsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-26 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.958, 61.249, 74.149 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.220 - 0.810 |
R-factor | 0.112 |
Rwork | 0.109 |
R-free | 0.12000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sgt |
RMSD bond length | 0.020 |
RMSD bond angle | 2.032 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.380 | 0.830 |
High resolution limit [Å] | 0.790 | 0.790 |
Number of reflections | 234158 | |
Completeness [%] | 99.8 | 96.9 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.9 | 298 | 10 mM calcium chloride, 100 mM HEPES pH 7.0, 2.72 M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |