4M7F

Crystal structure of tetrameric fibrinogen-like recognition domain of FIBCD1 with bound ManNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9745
Spacegroup name	P 4
Unit cell lengths	119.540, 119.540, 44.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.461 - 2.100
R-factor	0.1939
Rwork	0.187
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Native FIBCD1 structure
Data reduction software	MOSFLM
Data scaling software	SCALA (3.1.4)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.461	53.461	2.210
High resolution limit [Å]	2.100	6.640	2.100
Rmerge	0.069	0.033	0.174
Total number of observations		5204	23101
Number of reflections	36910
<I/σ(I)>	12.6	18	4.2
Completeness [%]	99.8	99.1	100
Redundancy	4.2	4.2	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.7M Ammonium sulphate, 7% Dioxane, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K