4M7A

Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	117.624, 78.379, 140.565
Unit cell angles	90.00, 100.58, 90.00

Refinement procedure

Resolution	39.189 - 2.781
R-factor	0.2465
Rwork	0.245
R-free	0.27840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m78
RMSD bond length	0.009
RMSD bond angle	1.269
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.900
High resolution limit [Å]	2.781	2.781
Number of reflections	31170
Completeness [%]	98.4	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K