4M68
Crystal structure of the mouse MLKL kinase-like domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-05 |
Detector | RAYONIX MX325HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.970, 61.522, 60.074 |
Unit cell angles | 90.00, 100.29, 90.00 |
Refinement procedure
Resolution | 27.916 - 1.696 |
R-factor | 0.1947 |
Rwork | 0.194 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m67 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.024 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 39125 | |
Completeness [%] | 97.9 | 86.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.3M potassium formate, 13% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |