4M5W
Crystal structure of the USP7/HAUSP catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.976 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.312, 69.193, 75.726 |
Unit cell angles | 90.00, 131.28, 90.00 |
Refinement procedure
Resolution | 38.441 - 2.244 |
R-factor | 0.1884 |
Rwork | 0.186 |
R-free | 0.24160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nb8 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.8.1_1168) |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.904 | 2.290 |
High resolution limit [Å] | 2.244 | 2.244 |
Rmerge | 0.077 | 0.549 |
Number of reflections | 17493 | |
<I/σ(I)> | 19.9 | 2.1 |
Completeness [%] | 92.6 | 66.4 |
Redundancy | 3.5 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 285 | 0.1 M HEPES, pH 7.5, 22% PEG3350, 0.2 M sodium bromide, 0.15 mM Cymal-7, VAPOR DIFFUSION, SITTING DROP, temperature 285K |