4M5A
Crystal structure of the complex of Ribosome inactivating protein from Momordica balsamina inhibited by asymmetric dimethyl arginine at 1.70 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-07-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | H 3 |
Unit cell lengths | 130.257, 130.257, 39.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.510 - 1.700 |
R-factor | 0.20808 |
Rwork | 0.201 |
R-free | 0.24084 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s9q |
RMSD bond length | 0.006 |
RMSD bond angle | 1.211 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.069 | 0.596 |
Number of reflections | 26101 | |
<I/σ(I)> | 23 | 5 |
Completeness [%] | 100.0 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 14% PEG 6000, 0.1M Sodium Phosphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |