4M59

Crystal structure of the pentatricopeptide repeat protein PPR10 in complex with an 18-nt psaJ RNA element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9067
Spacegroup name	P 43
Unit cell lengths	83.428, 83.428, 226.913
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.870 - 2.460
R-factor	0.261
Rwork	0.260
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m57
RMSD bond length	0.015
RMSD bond angle	0.999
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.540
High resolution limit [Å]	2.450	2.450
Number of reflections	54087
Completeness [%]	96.3	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	10% PEG 3350, 8% Tacsimate, 0.1M MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K