4M58

Crystal Structure of an transition metal transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 64 2 2
Unit cell lengths	122.727, 122.727, 125.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.056 - 3.210
R-factor	0.247
Rwork	0.245
R-free	0.28740
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	2.012
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.060
High resolution limit [Å]	3.200	3.200
Number of reflections	9403
Completeness [%]	97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	291.15	38% PEG 300, 0.05M Glycine, 0.1M sodium chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K