4M56
The Structure of Wild-type MalL from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-17 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9536 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.650, 100.260, 101.490 |
Unit cell angles | 90.00, 103.28, 90.00 |
Refinement procedure
Resolution | 32.950 - 2.300 |
R-factor | 0.19 |
Rwork | 0.187 |
R-free | 0.24500 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.416 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.1.27) |
Phasing software | PHASER (2.5.1) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 98.776 | 34.190 | 2.370 |
High resolution limit [Å] | 2.300 | 9.480 | 2.300 |
Rmerge | 0.159 | 0.085 | 0.553 |
Number of reflections | 51131 | ||
<I/σ(I)> | 4.9 | 9 | 1.7 |
Completeness [%] | 95.7 | 97.5 | 86.8 |
Redundancy | 2.8 | 2.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 200 mM LiSO4, 20% (w/v) PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |