4M4Z

SH3 and SH2 domains of human Src-like adaptor protein 2 (SLAP2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-11
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	38.460, 42.430, 46.100
Unit cell angles	90.00, 94.63, 90.00

Refinement procedure

Resolution	28.445 - 2.100
R-factor	0.1839
Rwork	0.182
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lck
RMSD bond length	0.004
RMSD bond angle	0.757
Data reduction software	MOSFLM
Data scaling software	SCALA (0.1.29)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.170	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.068	0.243
Number of reflections	8539
<I/σ(I)>	10.5	4.8
Completeness [%]	97.5	95.5
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	0.2 M Lithium chloride, 20% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K