4M4P
Crystal structure of EPHA4 ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 |
| Unit cell lengths | 112.589, 112.589, 49.552 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.000 - 2.081 |
| R-factor | 0.1904 |
| Rwork | 0.189 |
| R-free | 0.22490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fl7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.355 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 2.140 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.680 | |
| Number of reflections | 42061 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 22% PEG400, 0.1M MES buffer pH 6.0, 3% DMSO, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
