4M4M
The structure of Ni T6 bovine insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.040 |
| Spacegroup name | H 3 |
| Unit cell lengths | 80.850, 80.850, 33.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.780 - 1.500 |
| R-factor | 0.1842 |
| Rwork | 0.182 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.484 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 2010_07_29_2140)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.783 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 12710 | |
| Completeness [%] | 97.6 | 79.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.05M sodium citrate, 15%(v/v) acetone, 15mM nickel(II)acetate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
