4M4D
Crystal structure of lipopolysaccharide binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00599 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.282, 59.906, 119.932 |
Unit cell angles | 90.00, 102.63, 90.00 |
Refinement procedure
Resolution | 19.891 - 2.909 |
R-factor | 0.2322 |
Rwork | 0.230 |
R-free | 0.27720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ewf |
RMSD bond length | 0.012 |
RMSD bond angle | 1.671 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.068 | 0.272 |
Number of reflections | 21741 | |
<I/σ(I)> | 17.3 | 3.29 |
Completeness [%] | 91.4 | 76.9 |
Redundancy | 3.2 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 22% PEG 8000, 100mM MOPS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |