4M4C
Crystal structure of Rhodostomin ARGDP mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.619, 42.637, 46.538 |
| Unit cell angles | 106.84, 103.86, 97.23 |
Refinement procedure
| Resolution | 23.580 - 1.800 |
| R-factor | 0.18057 |
| Rwork | 0.177 |
| R-free | 0.23787 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uci |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.257 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 0.459 |
| Number of reflections | 19633 | |
| <I/σ(I)> | 23.9 | 2.3 |
| Completeness [%] | 96.7 | 91.8 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG4000, 0.2M Ammonium sulfate, 5% PEG3350, 2% PEG200, 0.5% 2-propanol(External) , pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
