4M4C
Crystal structure of Rhodostomin ARGDP mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-02 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97622 |
Spacegroup name | P 1 |
Unit cell lengths | 31.619, 42.637, 46.538 |
Unit cell angles | 106.84, 103.86, 97.23 |
Refinement procedure
Resolution | 23.580 - 1.800 |
R-factor | 0.18057 |
Rwork | 0.177 |
R-free | 0.23787 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uci |
RMSD bond length | 0.027 |
RMSD bond angle | 2.257 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.059 | 0.459 |
Number of reflections | 19633 | |
<I/σ(I)> | 23.9 | 2.3 |
Completeness [%] | 96.7 | 91.8 |
Redundancy | 3.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG4000, 0.2M Ammonium sulfate, 5% PEG3350, 2% PEG200, 0.5% 2-propanol(External) , pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |