4M43
Crystal structure of anti-rabies glycoprotein Fab 523-11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-12-05 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 85.431, 123.147, 42.456 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.598 - 1.850 |
R-factor | 0.196 |
Rwork | 0.194 |
R-free | 0.23220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1igi |
RMSD bond length | 0.011 |
RMSD bond angle | 1.359 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.600 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.079 | 0.511 |
Number of reflections | 39028 | 1965 |
<I/σ(I)> | 16.4 | 2.9 |
Completeness [%] | 100.0 | 99.6 |
Redundancy | 4.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 1.5M ammonium sulfate, 0.1M imidazole malate, 10% ethanol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |