4M3H
Crystal structure of small laccase Ssl1 from Streptomyces sviceus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9144 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.540, 103.820, 162.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.970 - 2.200 |
R-factor | 0.20913 |
Rwork | 0.206 |
R-free | 0.26803 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cg8 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.837 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.970 | 2.300 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.064 | 0.425 |
Number of reflections | 49366 | |
<I/σ(I)> | 17.3 | 3.27 |
Completeness [%] | 99.5 | 98.7 |
Redundancy | 4.4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 50 mM HEPES, pH 7.0, 1.0-1.3 M ammonium sulfate, 5-20 mM hexamminecobalt(III) chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |