4M3H

Crystal structure of small laccase Ssl1 from Streptomyces sviceus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9144
Spacegroup name	P 21 21 21
Unit cell lengths	51.540, 103.820, 162.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.970 - 2.200
R-factor	0.20913
Rwork	0.206
R-free	0.26803
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cg8
RMSD bond length	0.020
RMSD bond angle	1.837
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.970	2.300
High resolution limit [Å]	2.130	2.130
Rmerge	0.064	0.425
Number of reflections	49366
<I/σ(I)>	17.3	3.27
Completeness [%]	99.5	98.7
Redundancy	4.4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	50 mM HEPES, pH 7.0, 1.0-1.3 M ammonium sulfate, 5-20 mM hexamminecobalt(III) chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K