4M3C
Structure of a binary complex between homologous tetrameric legume lectins from Butea monosperma and Spatholobus parviflorus seeds
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-02-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.110, 78.630, 96.190 |
| Unit cell angles | 96.00, 89.99, 100.37 |
Refinement procedure
| Resolution | 15.840 - 2.500 |
| R-factor | 0.2151 |
| Rwork | 0.211 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.888 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto (MR) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.840 | |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 69131 | |
| Completeness [%] | 95.0 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 294 | 25% PEG-8000, 5% MPD, Phosphate buffer, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
