4M33
Crystal structure of gated-pore mutant H141D of second DNA-Binding protein under starvation from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-01-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 90.670, 90.670, 419.691 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.220 |
| R-factor | 0.17741 |
| Rwork | 0.175 |
| R-free | 0.21336 |
| Structure solution method | Direct refinement against model PDB |
| Starting model (for MR) | 2z90 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.892 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.134 | 0.328 |
| Number of reflections | 33223 | |
| <I/σ(I)> | 15.6 | 6.9 |
| Redundancy | 11.1 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil | 6.5 | 298 | 250mM MgCl2, 0.1M sodium cacodylate, 20% PEG3350, pH 6.5, Microbatch under oil, temperature 298K |
