4M2F
Crystal structure of non-heme iron oxygenase OrfP in complex with Fe and L-canavanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-07-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.993, 116.608, 96.177 |
| Unit cell angles | 90.00, 92.19, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.920 |
| R-factor | 0.21018 |
| Rwork | 0.208 |
| R-free | 0.24791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m23 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.194 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 107986 | |
| Completeness [%] | 99.3 | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 100mM Bis Tris Propane, 200mM NaF, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, temperature 293K |
