4M29
Structure of a GH39 Beta-xylosidase from Caulobacter crescentus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.60 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.287, 57.347, 94.050 |
Unit cell angles | 90.00, 91.38, 90.00 |
Refinement procedure
Resolution | 17.400 - 2.100 |
R-factor | 0.231 |
Rwork | 0.228 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ekj |
RMSD bond length | 0.016 |
RMSD bond angle | 1.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.400 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 25082 | |
Completeness [%] | 96.2 | 96.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M MES, 12% PEG6000, 0.05 M NaCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |