4M28
UDP-Glucose Pyrophosphorylase from Leishmania major in complex with UTP analog dUpCpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.250, 108.470, 152.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.270 - 3.000 |
| R-factor | 0.21642 |
| Rwork | 0.213 |
| R-free | 0.27914 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oef |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.300 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 11151 |
| <I/σ(I)> | 11.6 |
| Completeness [%] | 90.0 |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293.15 | 0.1 M MES, 20% w/v PEG-4000, 200 mM Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K, pH 6.4 |
