4M1X
Tetrameric ring structure of 201phi2-1p060 from Pseudomonas phage 201phi2-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1158, 1.771203 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.098, 52.672, 62.302 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.703 - 1.300 |
| R-factor | 0.1196 |
| Rwork | 0.119 |
| R-free | 0.14400 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.288 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 70298 | |
| <I/σ(I)> | 24.96 | 3.92 |
| Completeness [%] | 97.5 | 80.2 |
| Redundancy | 4.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 0.1M Tris pH 8.5, 0.3M NaCl, and 25% PEG3350 , VAPOR DIFFUSION, HANGING DROP, temperature 296K |
