4M1P
Crystal structure of the copper-sensing repressor CsoR with Cu(I) from Geobacillus thermodenitrificans NG80-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2013-05-09 |
Detector | NOIR-1 |
Wavelength(s) | 1.0 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 89.204, 89.204, 57.985 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.375 - 2.564 |
R-factor | 0.2173 |
Rwork | 0.214 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4adz |
RMSD bond length | 0.005 |
RMSD bond angle | 0.750 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.610 |
High resolution limit [Å] | 2.570 | 2.570 |
Rmerge | 0.067 | 0.800 |
Number of reflections | 4463 | |
<I/σ(I)> | 30.91 | 1.94 |
Completeness [%] | 99.8 | 100 |
Redundancy | 11.3 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 2.8M sodium formate, 0.1M HEPES, 0.1M betaine hydrochloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |