4M1H
X-ray crystal structure of Chlamydia trachomatis apo NrdB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2012-04-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.975, 97.394, 99.237 |
| Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
| Resolution | 29.748 - 1.695 |
| R-factor | 0.1809 |
| Rwork | 0.179 |
| R-free | 0.21030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1syy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.015 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.748 | |
| High resolution limit [Å] | 1.695 | 1.695 |
| Number of reflections | 155357 | |
| <I/σ(I)> | 28.6 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M sodium acetate, 10% PEG3000, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
