4M0V
Crystal structure of E.coli SbcD with Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Collection date | 2013-06-20 |
Wavelength(s) | 0.97880 |
Spacegroup name | P 1 |
Unit cell lengths | 62.162, 69.229, 95.187 |
Unit cell angles | 72.24, 84.00, 83.48 |
Refinement procedure
Resolution | 19.967 - 1.830 |
R-factor | 0.1636 |
Rwork | 0.162 |
R-free | 0.19860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lty |
RMSD bond length | 0.007 |
RMSD bond angle | 1.132 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Number of reflections | 128912 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |