4M0I
CRYSTAL STRUCTURE OF SYNTHETIC HIV-1 CAPSID C-TERMINAL DOMAIN (CTD) C198S mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-04 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97530 |
Spacegroup name | I 41 |
Unit cell lengths | 59.001, 59.001, 61.176 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.610 - 2.800 |
R-factor | 0.23893 |
Rwork | 0.236 |
R-free | 0.29379 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lry |
RMSD bond length | 0.012 |
RMSD bond angle | 1.573 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.057 | 0.669 |
Number of reflections | 2648 | |
<I/σ(I)> | 30.7 | 2.2 |
Completeness [%] | 99.1 | 99.3 |
Redundancy | 6.4 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 2 M ammonium sulfate, 100 mM sodium cacodylate pH 6.5, and 200 mM sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 294K |