4M0G
The crystal structure of an adenylosuccinate synthetase from Bacillus anthracis str. Ames Ancestor.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940, 0.97957 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 76.498, 76.498, 342.797 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.742 - 2.152 |
| R-factor | 0.1942 |
| Rwork | 0.192 |
| R-free | 0.24300 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.037 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.099 | 0.643 |
| Number of reflections | 55297 | |
| <I/σ(I)> | 40.2 | 4.3 |
| Completeness [%] | 97.3 | 99.2 |
| Redundancy | 9.1 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 25% (w/v) PEG1500, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
