4M02
Middle fragment(residues 494-663) of the binding region of SraP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97919 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.984, 46.461, 47.594 |
Unit cell angles | 90.00, 102.64, 90.00 |
Refinement procedure
Resolution | 46.440 - 1.590 |
R-factor | 0.16576 |
Rwork | 0.164 |
R-free | 0.20208 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m01 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.220 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.640 |
High resolution limit [Å] | 1.590 | 1.600 |
Rmerge | 0.103 | 0.323 |
Number of reflections | 18581 | |
<I/σ(I)> | 10.034 | 3.726 |
Completeness [%] | 99.6 | 99.1 |
Redundancy | 3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1M Tris, 2.0M Ammonium phosphate monobasic, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |