4M01
N terminal fragment(residues 245-575) of binding region of SraP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.704, 179.644, 71.510 |
Unit cell angles | 90.00, 89.77, 90.00 |
Refinement procedure
Resolution | 44.911 - 2.100 |
R-factor | 0.1596 |
Rwork | 0.158 |
R-free | 0.19510 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.337 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.158 | 0.357 |
Number of reflections | 99475 | |
<I/σ(I)> | 14.346 | 5.63 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.9 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 10% PEG 6000, 0.1M MES, 1.0M lithium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |