4LZX
Complex of IQCG and Ca2+-free CaM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 83 |
| Detector technology | CCD |
| Collection date | 2012-05-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.577, 60.922, 64.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.270 - 1.500 |
| R-factor | 0.1545 |
| Rwork | 0.153 |
| R-free | 0.18690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.197 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.081 | 0.514 |
| Number of reflections | 28793 | |
| <I/σ(I)> | 29.1 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13.8 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.6M ammonium sulfate, 1M lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
