4LZV

Bovine beta-lactoglobulin crystallized in the presence of 20 mM zinc chloride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2380
Spacegroup name	P 32 2 1
Unit cell lengths	89.820, 89.820, 40.750
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.890 - 2.440
R-factor	0.20721
Rwork	0.204
R-free	0.23316
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ph5
RMSD bond length	0.004
RMSD bond angle	0.871
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0025)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.890	2.500
High resolution limit [Å]	2.440	2.440
Number of reflections	7223
<I/σ(I)>	30.57	14.86
Completeness [%]	99.4	93.9
Redundancy	8.4	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH	5.9	277	20 mM zinc chloride, pH 5.9, BATCH, temperature 277K