4LZM

COMPARISON OF THE CRYSTAL STRUCTURE OF BACTERIOPHAGE T4 LYSOZYME AT LOW, MEDIUM, AND HIGH IONIC STRENGTHS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Temperature [K]	298
Detector technology	FILM
Detector	OSCILLATION CAMERA
Spacegroup name	P 32 2 1
Unit cell lengths	61.200, 61.200, 96.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.700
R-factor	0.165
RMSD bond length	0.016
RMSD bond angle	2.240
Data scaling software	AGROVATA / ROTAVATA
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.700 *
Rmerge	0.091 *
Total number of observations	61379 *
Number of reflections	19390 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	T4 lysozyme	25 (mg/ml)
2	1	drop	Tris-HCl	0.1 (M)
3	1	reservoir		0.4 (M)
4	1	reservoir	PEG3500	30 (%)
5	1	reservoir	2-mercaptoethanol	14 (mM)