4LZL
Structure of the inactive form of the regulatory domain from the repressor of iron transport regulator (RitR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.460, 33.840, 34.960 |
| Unit cell angles | 92.65, 103.71, 97.53 |
Refinement procedure
| Resolution | 27.352 - 1.550 |
| R-factor | 0.1431 |
| Rwork | 0.141 |
| R-free | 0.16140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mvo |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.204 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.850 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.054 | 0.090 |
| Number of reflections | 17289 | |
| <I/σ(I)> | 12.5 | 7.3 |
| Completeness [%] | 94.6 | 93 |
| Redundancy | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1ul 5-10 mg/ml protein in 10.0 mM BIS-TRIS propane, pH 7.5 plus 1ul of 20-25% polyethylene glycol (PEG) 3350, 2.5 mM magnesium formate, and 20.0 mM TRIS, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
