4LZI

Characterization of Solanum tuberosum Multicystatin and Significance of Core Domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	65.940, 65.940, 191.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.121 - 2.200
R-factor	0.2219
Rwork	0.219
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w9p
RMSD bond length	0.009
RMSD bond angle	1.241
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.210	25.210	1.540
High resolution limit [Å]	1.490	3.210	1.490
Number of reflections	51806
Completeness [%]	73.1	97.7	73.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	277	0.4M Ammonium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 277K