4LZF
A novel domain in the microcephaly protein CPAP suggests a role in centriole architecture
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.580, 38.390, 70.050 |
| Unit cell angles | 90.00, 126.68, 90.00 |
Refinement procedure
| Resolution | 48.420 - 1.720 |
| R-factor | 0.1917 |
| Rwork | 0.190 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ld1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.114 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.29) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.420 | 48.420 | 1.760 |
| High resolution limit [Å] | 1.720 | 7.690 | 1.720 |
| Rmerge | 0.034 | 0.032 | 0.403 |
| Total number of observations | 695 | 1649 | |
| Number of reflections | 20094 | ||
| <I/σ(I)> | 19.5 | 36.5 | 2.1 |
| Completeness [%] | 74.4 | 78.6 | 31.8 |
| Redundancy | 1.3 | 1.4 | 0.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
